Process and Genes for Expression and Overexpression of Active [FeFe] Hydrogenases. U.S. Patent No. 8,835,153.
Author: Seibert, M.; King, P. W.; Ghirardi, M. L.; Posewitz, M. C.; Smolinski, S. L.
Publication Year: 2014
Notes: Assignee: Alliance for Sustainable Energy, LLC (Golden, CO).
Document Type: Patent
Subject Code Description: Chemical and Biosciences
Abstract: A process for expression of active [FeFe]-hydrogenase in a host organism that does not contain either the structural gene(s) for [FeFe]-hydrogenases and/or homologues for the maturation genes HydE, HydF and HyG, comprising: cloning the structural hydrogenase gene(s) and/or the maturation genes HydE, HydF and HydG from an organisms that contains these genes into expression plasmids; transferring the plasmids into an organism that lacks a native [FeFe]-hydrogenase or that has a disrupted [FeFe]-hydrogenase and culturing it aerobically; and inducing anaerobiosis to provide [FeFe] hydrogenase biosynthesis and H?2#191 production.
Accession Number: 60922
Library Notes: XXX-1409 REV
Report Numbers: PT-2A00-62992