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Records 1 to 118 of 118

1.  Seibert, M.; King, P. W.; Ghirardi, M. L.; Posewitz, M. C.; Smolinski, S. L. (2014). Process and Genes for Expression and Overexpression of Active [FeFe] Hydrogenases. U.S. Patent No. 8,835,153.

2.  Wecker, M. S. A.; Ghirardi, M., L. (2014). High-Throughput Biosensor Discriminates Between Different Algal H2-Photoproducing Strains. Biotechnology and Bioengineering. Vol. 111(7), July 2014; pp. 1332-1340; NREL Report No. JA-2700-60470. http://dx.doi.org/10.1002/bit.25206

3.  King, P.; Ghirardi, M. L.; Seibert, M. (2014). Oxygen-Resistant Hydrogenases and Methods for Designing and Making Same. U.S. Patent No. 8,663,958.

4.  Peden, E. A.; Boehm, M.; Mulder, D. W.; Davis, R.; Old, W. M.; King, P. W.; Ghirardi, M. L.; Dubini, A. (2013). Identification of Global Ferredoxin Interaction Networks in Chlamydomonas reinhardtii. Journal of Biological Chemistry. Vol. 288(49), 6 December 2013; pp. 35192-35209; NREL Report No. JA-2700-60563. http://dx.doi.org/10.1074/jbc.M113.483727

5.  Shircliff, R. A.; Stradins, P.; Moutinho, H.; Fennell, J.; Ghirardi, M. L.; Cowley, S. W.; Branz, H. M.; Martin, I. T. (2013). Angle-Resolved XPS Analysis and Characterization of Monolayer and Multilayer Silane Films for DNA Coupling to Silica. Langmuir. Vol. 29(12), 26 March 2013; pp. 4057-4067; NREL Report No. JA-5200-58665. http://dx.doi.org/10.1021/la304719y


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6.  Ghirardi, M.; Svedruzic, D. (2013). Identification of Catalysts and Materials for a High-Energy Density Biochemical Fuel Cell: Cooperative Research and Development Final Report, CRADA Number CRD-09-345. 3 pp.; NREL Report No. TP-7A10-58457.

7.  Yang, S.; Guarnieri, M. T.; Smolinski, S.; Ghirardi, M.; Pienkos, P. T. (2013). De Novo Transcriptomic Analysis of Hydrogen Production in the Green Alga Chlamydomonas moewusii through RNA-Seq. Biotechnology for Biofuels. Vol. 6(6), June 2013; 17 pp.; NREL Report No. JA-5100-60226. http://dx.doi.org/10.1186/1754-6834-6-118

8.  Ghirardi, M. L. (2013). Photobiological H2 Production: Theoretical Maximum Light Conversion Efficiency and Strategies to Achieve It. Wu, N., Chiu, K. S., Chu, D., et al., eds. ECS Transactions. Papers from PRiME 2012, 7-12 October 2012, Honolulu, Hawaii. Vol. 50(49), 2013; pp. 47-50; NREL Report No. CP-2700-57353. http://dx.doi.org/10.1149/05049.0047ecst

9.  Ungerer, J.; Tao, L.; Davis, M.; Ghirardi, M.; Maness, P. C.; Yu, J. (2012). Sustained Photosynthetic Conversion of CO2 to Ethylene in Recombinant Cyanobacterium Synechocystis 6803. Energy and Environmental Science. Vol. 5(10), October 2012; pp. 8998-9006; NREL Report No. JA-2700-53018. http://dx.doi.org/10.1039/C2EE22555G


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10.  Ghirardi, M. L. (2012). Biological Systems for Hydrogen Photoproduction (Presentation). NREL (National Renewable Energy Laboratory). 16 pp.; NREL Report No. PR-2700-54467.

11.  Kosourov, S. N.; Batyrova, K. A.; Petushkova, E. P.; Tsygankov, A. A.; Ghirardi, M. L.; Seibert, M. (2012). Maximizing the Hydrogen Photoproduction Yields in Chlamydomonas Reinhardtii Cultures: The Effect of the H2 Partial Pressure. International Journal of Hydrogen Energy. Vol. 37(10), May 2012; pp. 8850-8858; NREL Report No. JA-2700-54079. http://dx.doi.org/10.1016/j.ijhydene.2012.01.082

12.  Narayana Murthy, U. M.; Wecker, M. S. A.; Posewitz, M. C.; Gilles-Gonzalez, M. A.; Ghirardi, M. L. (2012). Novel FixL Homologues in Chlamydomonas reinhardtii Bind Heme and 02. FEBS Letters. Vol. 586(24), 14 December 2012; pp. 4282-4288; NREL Report No. JA-2700-55904. http://dx.doi.org/10.1016/j.febslet.2012.06.052

13.  Kosourov, S. N.; Ghirardi, M. L.; Seibert, M. (2011). Truncated Antenna Mutant of Chlamydomonas Reinhardtii Can Produce More Hydrogen than the Parental Strain. International Journal of Hydrogen Energy. Vol. 36(3), February 2011; pp. 2044-2048; NREL Report No. JA-2A00-49715. http://dx.doi.org/10.1016/j.ijhydene.2010.10.041

14.  Blankenship, R. E.; Tiede, D. M.; Barber, J.; Brudvig, G. W.; Fleming, G.; Ghirardi, M.; Gunner, M. R.; Junge, W.; Kramer, D. M.; Melis, A.; Moore, T. A.; Moser, C. C.; Nocera, D. G.; Nozik, A. J.; Ort, D. R.; Parson, W. W.; Prince, R. C.; Sayre, R. T. (2011). Comparing Photosynthetic and Photovoltaic Efficiencies and Recognizing the Potential for Improvement. Science. Vol. 332(6031), 13 May 2011; pp. 805-809; NREL Report No. JA-2700-50896. http://dx.doi.org/10.1126/science.1200165

15.  Yacoby, I.; Pochekailov, S.; Toporik, H.; Ghirardi, M. L.; King, P. W.; Zhang, S. G. (2011). Photosynthetic Electron Partitioning Between [FeFe] Hydrogenase and Ferredoxin: NADP+-Oxidoreductase (FNR) Enzymes in Vitro. Proceedings of the National Academy of Sciences of the United States of America. Vol. 108(23), 7 June 2011; pp. 9396-9401; NREL Report No. JA-2700-51092. http://dx.doi.org/10.1073/pnas.1103659108

16.  Wecker, M. S. A.; Meuser, J. E.; Posewitz, M. C.; Ghirardi, M. L. (2011). Design of a New Biosensor for Algal H2 Production Based on the H2-Sensing System of Rhodobacter capsulatus. International Journal of Hydrogen Energy. Vol. 36(17), August 2011; 11229-11237; NREL Report No. JA-2700-51929. http://dx.doi.org/10.1016/j.ijhydene.2011.05.121

17.  Shircliff, R. A.; Martin, I. T.; Pankow, J. W.; Fennell, J.; Stradins, P.; Ghirardi, M. L.; Cowley, S. W.; Branz, H. M. (2011). High-Resolution X-Ray Photoelectron Spectroscopy of Mixed Silane Monolayers for DNA Attachment. ACS Applied Materials and Interfaces. Vol. 3(9), 28 September 2011; pp. 3285-3292; NREL Report No. JA-5200-53364. http://dx.doi.org/10.1021/am200604q

18.  Meuser, J. E.; Boyd, E. S.; Ananyev, G.; Karns, D.; Radakovits, R.; Murthy, U. M. N.; Ghirardi, M. L.; Dismukes, G. C.; Peters, J. W.; Posewitz, M. C. (2011). Evolutionary Significance of an Algal Gene Encoding an [FeFe]-Hydrogenase with F-Domain Homology and Hydrogenase Activity in Chlorella Variabilis NC64A. Planta. Vol. 234(4), October 2011; pp. 829-843; NREL Report No. JA-2700-54258. http://dx.doi.org/10.1007/s00425-011-1431-y

19.  Jorquera, O.; Kiperstok, A.; Sales, E. A.; Embirucu, M.; Ghirardi, M. L. (2010). Comparative Energy Life-Cycle Analyses of Microalgal Biomass Production in Open Ponds and Photobioreactors. Bioresource Technology. Vol. 101(4), 2010; pp. 1406-1413; NREL Report No. JA-270-47226. http://dx.doi.org/10.1016/j.biortech.2009.09.038


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20.  Ghirardi, M. (2010). Biological Systems for Hydrogen Photoproduction (Presentation). 24 pp.; NREL Report No. PR-270-48068.

21.  Ghirardi, M. L.; Kosourov, S.; Maness, P.; Smolinski, S.; Seibert, M. (2010). Algal Hydrogen Production. Flickinger, M. C., ed. Encyclopedia of Industrial Biotechnology: Bioprocess, Bioseparation, and Cell Technology. Hoboken, NJ: John Wiley and Sons, Inc. pp. 184-198; NREL Report No. CH-270-45637.

22.  Kosourov, S.; Ghirardi, M. L.; Seibert, M. (2010). Multi-Stage Microbial System for Continuous Hydrogen Production. U.S. Patent No. 7,732,174. 13 pp.; NREL Report No. PT-2700-51064.

23.  Ghirardi, M. L.; Mohanty, P. (2010). Oxygenic Hydrogen Photoproduction - Current Status of the Technology. Current Science. Review Article. Vol. 98(4), 25 February 2010; pp. 499-507; NREL Report No. JA-270-47482.

24.  Belokopytov, B. F.; Laurinavichius, K. S.; Laurinavichene, T. V.; Ghirardi, M. L.; Seibert, M.; Tsygankov, A. A. (2009). Towards the Integration of Dark- and Photo-Fermentative Waste Treatment. 2. Optimization of Starch-Dependent Fermentative Hydrogen Production. International Journal of Hydrogen Energy. Vol. 34(8), 2009; pp. 3324-3332; NREL Report No. JA-270-45467. http://dx.doi.org/10.1016/j.ijhydene.2009.02.042

25.  Meuser, J. E.; Ananyev, G.; Wittig, L. E.; Kosourov, S.; Ghirardi, M. L.; Seibert, M.; Dismukes, G. C.; Posewitz, M. C. (2009). Phenotypic Diversity of Hydrogen Production in Chlorophycean Algae Reflects Distinct Anaerobic Metabolisms. Journal of Biotechnology. Vol. 142(1), 2009; pp. 21-30; NREL Report No. JA-270-46135. http://dx.doi.org/10.1016/j.jbiotec.2009.01.015

26.  Ghirardi, M. L.; Dubini, A.; Yu, J.; Maness, P. C. (2009). Photobiological Hydrogen-Producing Systems. Chemical Society Reviews. 2009 Renewable Energy Issue. Vol. 38(1), 2009; pp. 52-61; NREL Report No. JA-270-45097. http://dx.doi.org/10.1039/b718939g

27.  Posewitz, M. C.; Dubini, A.; Meuser, J.E.; Seibert, M.; Ghirardi, M. L. (2009). Chapter 7: Hydrogenases, Hydrogen Production, and Anoxia. Stern, D. B., ed. The Chlamydomonas Sourcebook, Second Edition. Volume 2: Organellar and Metabolic Processes. Oxford, UK: Academic Press pp. 217-255; NREL Report No. CH-270-43063.

28.  Maness, P. C.; Yu, J.; Eckert, C.; Ghirardi, M. L. (2009). Photobiological Hydrogen Production - Prospects and Challenges. Microbe Magazine. Vol. 4(6), June 2009; pp. 275-280; NREL Report No. JA-270-45182.

29.  Long, H.; King, P. W.; Ghirardi, M. L.; Kim, K. (2009). Hydrogenase/Ferredoxin Charge-Transfer Complexes: Effect of Hydrogenase Mutations on the Complex Association. Journal of Physical Chemistry A. Vol. 113(16), 2009; pp. 4060-4067; NREL Report No. JA-590-44322. http://dx.doi.org/10.1021/jp810409z

30.  King, P.; Ghirardi, M. L.; Seibert, M. (2009). Oxygen-Resistant Hydrogenases and Methods for Designing and Making Same. U.S. Patent No. 7,501,270 B2. 23 pp.; NREL Report No. PT-270-45830.

31.  Ghirardi, M. L.; Maness, P. C.; Seibert, M. (2008). Chapter 8: Photobiological Methods of Renewable Hydrogen Production. Rajeshwar, K.; McConnell, R.; Licht, S., eds. Solar Hydrogen Generation: Toward a Renewable Energy Future. New York, NY: Springer pp. 229-271; NREL Report No. CH-270-44205.

32.  Turner, J.; Sverdrup, G.; Mann, M. K.; Maness, P.-C.; Kroposki, B.; Ghirardi, M.; Evans, R. J.; Blake, D. (2008). Renewable Hydrogen Production. International Journal of Energy Research. Vol. 32(5), 2008; pp. 379-407; NREL Report No. JA-560-42271. http://dx.doi.org/10.1002/er.1372

33.  Hu, Q.; Sommerfeld, M.; Jarvis, E.; Ghirardi, M.; Posewitz, M; Seibert, M.; Darzins, A. (2008). Microalgal Triacylglycerols as Feedstocks for Biofuel Production: Perspectives and Advances. The Plant Journal. Vol. 54, 2008; pp. 621-639; NREL Report No. JA-510-42685. http://dx.doi.org/10.1111/j.1365-313X.2008.03492.x;

34.  Sverdrup, G. M.; Turner, J. A.; Mann, M. K.; Maness, P. C.; Kroposki, B.; Ghirardi, M. L.; Evans, R. J.; Blake, D. (2008). Status of Renewable Hydrogen Production in the United States. Abstract No. PETR-69. American Chemical Society. Abstracts of Papers of the 235th ACS National Meeting, 6-8 April 2008, New Orleans, LA. Washington, DC: American Chemical Society (ACS) 1 p.; NREL Report No. AB-4A0-49032.

35.  Long, H.; Chang, C. H.; King, P. W.; Ghirardi, M. L.; Kim, K. (2008). Brownian Dynamics and Molecular Dynamics Study of the Association Between Hydrogenase and Ferredoxin from the Chlamydomonas reinhardtii. Biophysical Journal. Vol. 95, October 2008; pp. 3753-3766; NREL Report No. JA-530-41890. http://dx.doi.org/10.1529/biophysj.107.127548

36.  Laurinavichene, T. V.; Kosourov, S. N.; Ghirardi, M. L.; Seibert, M.; Tsygankov, A. A. (2008). Prolongation of H2 Photoproduction by Immobilized, Sulfur-Limited Chlamydomonas reinhardtii Cultures. Journal of Biotechnology. Vol. 134(3-4), 30 April 2008; pp. 275-277; NREL Report No. JA-270-42722. http://dx.doi.org/10.1016/j.ijhydene.2005.05.002

37.  Laurinavichene, T. V.; Tekucheva, D. N.; Laurinavichius, K. S.; Ghirardi, M. L.; Seibert, M.; Tsygankov, A. A. (2008). Towards the Integration of Dark and Photo Fermentative Waste Treatment. 1. Hydrogen Photoproduction by Purple Bacterium Rhodobacter capsulatus using Potential Products of Starch Fermentation. International Journal of Hydrogen Energy. Vol. 33(23), December 2008; pp. 7020-7026; NREL Report No. JA-270-45100. http://dx.doi.org/10.1016/j.ijhydene.2008.09.033

38.  Hambourger, M.; Gervaldo, M.; Svedruzic, D.; King, P. W.; Gust, D.; Ghirardi, M.; Moore, A. L.; Moore, T. A. (2008). [FeFe]-Hydrogenase-Catalyzed H2 Production in a Photoelectrochemical Biofuel Cell. Journal of the American Chemical Society. Vol. 130(6), 2008; pp. 2015-2022; NREL Report No. JA-270-42429. http://dx.doi.org/10.1021/ja077691k

39.  Seibert, M.; King, P. W.; Posewitz, M. C.; Melis, A.; Ghirardi, M. L. (2008). Chapter 22: Photosynthetic Water-Splitting for Hydrogen Production. Wall, J. D., Harwood, C. S., Demain, A., eds. Bioenergy. Washington, D.C.: ASM Press pp. 273-291; NREL Report No. JA-270-42871.

40.  Blake, D. M.; Amos, W. A.; Ghirardi, M. L.; Seibert, M. (2008). Chapter 5: Materials Requirements for Photobiological Hydrogen Production. Jones, R. H.; Thomas, G. J., eds. Materials for the Hydrogen Economy. Boca Raton, FL: CRC Press pp. 123-145; NREL Report No. CH-510-39199.

41.  Jorquera, O.; Kiperstok, A.; Sales, E. A.; Emribirucu, M.; Ghirardi, M. L. (2008). S-Systems Sensitivity Analysis of the Factors that May Influence Hydrogen Production by Sulfur-Deprived Chlamydomnas reinhardtii. International Journal of Hydrogen Energy. Vol. 33(9), 2008; pp. 2167-2177; NREL Report No. JA-270-42812. http://dx.doi.org/10.1016/j.ijhydene.2008.01.054

42.  Chang, C. H.; King, P. W.; Ghirardi, M. L.; Kim, K. (2007). Atomic Resolution Modeling of the Ferredoxin:[FeFe] Hydrogenase Complex from Chlamydomonas reinhardtii. Biophysical Journal. Vol. 93, November 2007; pp. 3034-3045; NREL Report No. JA-530-41713. http://dx.doi.org/10.1529/biophysj.107.108589

43.  Nagy, L. E.; Meuser, J. E.; Plummer, S.; Seibert, M.; Ghirardi, M. L.; King, P. W.; Ahmann, D.; Posewitz, M. C. (2007). Application of Gene-Shuffling for the Rapid Generation of Novel [FeFe]-Hydrogenase Libraries. Biotechnology Letters. Vol. 29(3), 2007; pp. 421-430; NREL Report No. JA-270-41455. http://dx.doi.org/10.1007/s10529-006-9254-9

44.  Ghirardi, M. L.; Posewitz, M. C.; Maness, P. C.; Dubini, A.; Yu, J.; Seibert, M. (2007). Hydrogenases and Hydrogen Photoproduction in Oxygenic Photosynthetic Organisms. Annual Review of Plant Biology. Vol. 58, 2007; pp. 71-91; NREL Report No. JA-270-42244. http://dx.doi.org/10.1146/annurev.arplant.58.032806.103848

45.  Makarova, V. V.; Kosourov, S.; Krendeleva, T. E.; Semin, B. K.; Kukarskikh, G. P.; Rubin, A. B.; Sayre, R. T.; Ghirardi, M. L.; Seibert, M. (2007). Photoproduction of Hydrogen by Sulfur-Deprived Chlamydomonas reinhardtii Mutants with Impaired Photosystem II Photochemical Activity. Photosynthesis Research. Vol. 94(1), 2007; pp. 79-89; NREL Report No. JA-270-41147. http://dx.doi.org/10.1007/s11120-007-9219-4

46.  Kosourov, S.; Patrusheva, E.; Ghirardi, M. L.; Seibert, M.; Tsygankov, A. (2007). Comparison of Hydrogen Photoproduction by Sulfur-Deprived Chlamydomonas reinhardtii Under Different Growth Conditions. Journal of Biotechnology. Vol. 128(4), 2007; pp. 776-787; NREL Report No. JA-270-41145. http://dx.doi.org/10.1016/j.jbiotec.2006.12.025

47.  King, P. W.; Svedruzic, D.; Hambourger, M.; Gervaldo, M.; McDonald, T.; Blackburn, J.; Heben, M.; Gust, D.; Moore, A. L.; Moore, T. A.; Ghirardi, M. L. (2007). Merging [FeFe]-Hydrogenases with Materials and Nanomaterials as Biohybrid Catalysts for Solar H2 Production. Guo, J. ed. Solar Hydrogen and Nanotechnology II. Proceedings of SPIE, Vol. 6650. 7 pp.; NREL Report No. CP-270-42088. http://dx.doi.org/10.1117/12.736556


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48.  Ghirardi, M. L. (2007). Biological Systems for Hydrogen Photoproduction (Presentation). 13 pp.; NREL Report No. PR-510-41491.

49.  Hahn, J. J.; Ghirardi, M. L.; Jacoby, W. A. (2007). Immobilized Algal Cells Used for Hydrogen Production. Biochemical Engineering Journal. Vol. 37(1), 2007; pp. 75-79; NREL Report No. JA-270-42607. http://dx.doi.org/10.1016/j.bej.2007.03.010

50.  Laurinavichene, T. V.; Fedorov, A. S.; Ghirardi, M. L.; Seibert, M.; Tsygankov, A. A. (2006). Demonstration of Sustained Hydrogen Photoproduction by Immobilized, Sulfur-Deprived Chlamydomonas reinhardtii Cells. International Journal of Hydrogen Energy. Vol. 31(5), 2006; pp. 659-667; NREL Report No. JA-270-40256. http://dx.doi.org/10.1016/j.ijhydene.2005.05.002

51.  Ghirardi, M. L.; King, P.; Seibert, M. (2006). Chapter II.E.2: Biological Systems for Hydrogen Photoproduction. DOE Hydrogen Program 2006 Annual Progress Report. pp. 120-124; NREL Report No. CH-270-40266.

52.  Tsygankov, A. A.; Kosourov, S. N.; Tolstygina, I. V.; Ghirardi, M. L.; Seibert, M. (2006). Hydrogen Production by Sulfur-Deprived Chlamydomonas reinhardtii Under Photoautotrophic Conditions. International Journal of Hydrogen Energy. Vol. 31(11), September 2006; pp. 1574-1584; NREL Report No. JA-270-41025. http://dx.doi.org/10.1016/j.ijhydene.2006.06.024

53.  Ghirardi, M. L.; Cohen, J.; King, P.; Schulten, K.; Kim, K.; Seibert, M. (2006). [FeFe]-Hydrogenases and Photobiological Hydrogen Production. Vayssieres, L. Solar Hydrogen and Nanotechnology. Proceedings of SPIE, Vol. 6340. Vol. 6340, 2006; Bellingham, WA: SPIE 6 pp.; NREL Report No. CP-270-44065. http://dx.doi.org/10.1117/12.683767;

54.  King, P. W.; Posewitz, M. C.; Ghirardi, M. L.; Seibert, M. (2006). Functional Studies of [FeFe] Hydrogenase Maturation in an Escherichia coli Biosynthetic System. Journal of Bacteriology. Vol. 188(6), March 2006; pp. 2163-2172; NREL Report No. JA-590-40255. http://dx.doi.org/10.1128/JB.188.6.2163-2172.2006

55.  King, P. W.; Svedruzic, D.; Cohen, J.; Schulten, K.; Seibert, M.; Ghirardi, M. L. (2006). Structural and Functional Investigations of Biological Catalysts for Optimization of Solar-Driven, H2 Production Systems. Vayssieres, L. Solar Hydrogen and Nanotechnology. Proceedings of SPIE, Vol. 6340. Vol. 6340, 2006; Bellingham, WA: SPIE 9 pp.; NREL Report No. CP-270-44067. http://dx.doi.org/10.1117/12.693636;

56.  Ghirardi, M. L. (2006). Hydrogen Production by Photosynthetic Green Algae. Indian Journal of Biochemistry and Biophysics. Vol. 43, August 2006; pp. 201-210; NREL Report No. JA-270-41704.

57.  Melis, A.; Zhang, L.; Benemann, J. R.; Forestier, M.; Ghirardi, M.; Seibert, M. (2006). Hydrogen Production Using Hydrogenase-Containing Oxygenic Photosynthetic Organisms. U.S. Patent No. 6,989,252 B2. 12 pp.; NREL Report No. PT-590-39543.


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58.  Ghirardi, M.; King, P.; Maness, P. C.; Seibert, M. (2006). Biological Systems for Hydrogen Photoproduction (Poster). 24 pp.; NREL Report No. PO-560-39994.

59.  Cohen, J.; Kim, K.; Posewitz, M.; Ghirardi, M. L.; Schulten, K.; Seibert, M.; King, P. (2005). Molecular Dynamics and Experimental Investigation of H2 and O2 Diffusion in [Fe]-Hydrogenase. Biochemical Society Transactions. Vol. 33(Part 1), 2005; pp. 80-82; NREL Report No. JA-590-38440.

60.  Fedorov, A. S.; Laurinavichene, T. V.; Ghirardi, M. L.; Seibert, M.; Tsygankov, A. A. (2005). Demonstration of Sustained Hydrogen Photoproduction by Immobilized, Sulfur-Deprived Chlamydomonas reinhardtii Cells. 27th Symposium on Biotechnology for Fuels and Chemicals: Program and Abstracts (NREL/BK-510-36826), 1-4 May 2005, Denver, Colorado. Golden, CO: National Renewable Energy Laboratory p. 24; NREL Report No. AB-590-39813.

61.  Fedorov, A. S.; Kosourov, S.; Ghirardi, M. L.; Seibert, M. (2005). Continuous Hydrogen Photoproduction by Chlamydomonas reinhardtii. Davison, B. H., et al., eds. Applied Biochemistry and Biotechnology. Proceedings of the Twenty-Sixth Symposium on Biotechnology for Fuels and Chemicals, 9-12 May 2004, Chattanooga, Tennessee. Vol. 121-124, 2005; pp. 403-412; NREL Report No. JA-590-38273. http://dx.doi.org/10.1385/ABAB:121:1-3:0403

62.  Ghirardi, M. L.; King, P.; Kosourov, S.; Forestier, M.; Zhang, L.; Seibert, M. (2005). Chapter 11: Development of Algal Systems for Hydrogen Photoproduction; Addressing the Hydrogenase Oxygen-Sensitivity Problem. Collings, A. F.; Critchley, C. eds. Artificial Photosynthesis: From Basic Biology to Industrial Application. Weinheim, Germany: Wiley-VCH GmbH & Co. KGaA pp. 213-227; NREL Report No. CH-590-40692.

63.  Kosourov, S.; Makarova, V.; Fedorov, A. S.; Tsygankov, A.; Siebert, M.; Ghirardi, M. L. (2005). Effect of Sulfur Re-Addition on H2 Photoproduction by Sulfur-Deprived Green Algae. Photosynthesis Research. , 2005; pp. 295-305; NREL Report No. JA-590-39363. http://dx.doi.org/10.1007/s11120-005-5105-0


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64.  Ghirardi, M. L.; Kim, K.; King, P.; Maness, P. C.; Seibert, M. (2005). Biological Systems for Hydrogen Photoproduction (Presentation). 20 pp.; NREL Report No. PR-560-38183.

65.  Makarova, V. V.; Kosourov, S. N.; Krendeleva, T. E.; Kukarskikh, G. P.; Ghirardi, M. L.; Seibert, M.; Rubin, A. B. (2005). Photochemical Activity of Photosystem II and Hydrogen Photoproduction in Sulfur-Deprived Chlamydomonas reinhardtii Mutants D1-R323D and D1-R323L. (Russian). Biofizika. Vol. 50(6), 2005; pp. 1070-1078; NREL Report No. JA-590-40326.

66.  Posewitz, M. C.; King, P. W.; Smolinski, S. L.; Smith II, R. D.; Ginley, A. R.; Ghirardi, M. L.; Seibert, M. (2005). Identification of Genes Required for Hydrogenase Activity in Chlamydomonas reinhardtii. Biochemical Society Transactions. Vol. 33(Part 1), 2005; pp. 102-104; NREL Report No. JA-590-38657.


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67.  Ghirardi, M. L.; Maness, P. C.; Seibert, M. (2005). Chapter IV.E.2: Biological Systems for Hydrogen Photoproduction. DOE Hydrogen Program 2005 Annual Progress Report. pp. 216-224; NREL Report No. CH-560-38389.

68.  Ghirardi, M. L.; King, P. W.; Posewitz, M. C.; Maness, P. C.; Fedorov, A.; Kim, K.; Cohen, J.; Schulten, K.; Seibert, M. (2005). Approaches to Developing Biological H2-Photoproducing Organisms and Processes. Biochemical Society Transactions. Vol. 33(Part 1), 2005; pp. 70-72; NREL Report No. JA-590-38437.

69.  Hahn, J. J.; Ghirardi, M. L.; Jacoby, W. A. (2004). Effect of Process Variables on Photosynthetic Algal Hydrogen Production. Biotechnology Progress. Vol. 20, 2004; pp. 989-991; NREL Report No. JA-590-36903. http://dx.doi.org/10.1021/bp0341287

70.  Posewitz, M. C.; King, P. W.; Smolinski, S. L.; Zhang, L.; Seibert, M.; Ghirardi, M. L. (2004). Discovery of Two Novel Radical S-Adenosylmethionine Proteins Required for the Assembly of an Active [Fe] Hydrogenase. Journal of Biological Chemistry. Vol. 279(24), 11 June 2004; pp. 25711-25720; NREL Report No. JA-590-36919. http://dx.doi.org/10.1074/jbc.M403206200

71.  Posewitz, M. C.; Smolinski, S. L.; Kanakagiri, S.; Melis, A.; Seibet, A.; Ghirardi, M. L. (2004). Hydrogen Photoproduction Is Attenuated by Disruption of an Isoamylase Gene in Chlamydomonas reinhardtii. Plant Cell. Vol. 16(8), August 2004; pp. 2151-2163; NREL Report No. JA-590-36918. http://dx.doi.org/10.1105/tpc.104.021972

72.  Ghirardi, M. L.; Amos, W. (2004). Renewable Hydrogen from Green Algae. BioCycle. Vol. 45(5), May 2004; pp. 59+62; NREL Report No. JA-590-36955.

73.  Ghirardi, M. L.; Melis, A.; Lee, J. W.; Greenbaum, E.; Seibert, M. (2004). Photobiological H2 Production Research in the USA. A.A.M. Sayigh, ed. Proceedings of the World Renewable Energy Congress VIII (WREC 2004), 29 August--3 September 2004, Denver, Colorado (CD-ROM). [Amsterdam]: Elsevier, Ltd.; Monterey, CA: Produced by InControl Productions, Inc. 5 pp.; NREL Report No. CP-590-36855.

74.  Forestier, M.; King, P.; Zhang, L.; Posewitz, M.; Schwarzer, S.; Happe, T.; Ghirardi, M. L.; Seibert, M. (2003). Expression of two [Fe]-Hydrogenases in Chlamydomonas reinhardtii under Anaerobic Conditions. European Journal of Biochemistry. Vol. 270, 2003; pp. 2750-2758; NREL Report No. JA-590-34980. http://dx.doi.org/10.1046/j.1432-1033.2003.03656

75.  Kosourov, S.; Seibert, M.; Ghirardi, M. L. (2003). Effects of Extracellular pH on the Metabolic Pathways in Sulfur-Deprived H2-Producing Chlamydomonas Reinhardtii Cultures. Plant and Cell Physiology. Vol. 44(2), 2003; pp. 146-155; NREL Report No. JA-590-34437. http://dx.doi.org/10.1093/pcp/pcg020

76.  Seibert, M.; Ghirardi, M. (2003). Algal Hydrogen Production -- Physiology and Process Development. 25th Symposium on Biotechnology for Fuels and Chemicals: Program and Abstracts (NREL/BK-510-33708). Golden, CO: National Renewable Energy Laboratory p. 141; NREL Report No. AB-590-35099.

77.  Antal, T. K.; Krendeleva, T. E.; Laurinavichene, T. V.; Makarova, V. V.; Ghirardi, M. L.; Rubin, A. B.; Tsygankov, A. A.; Seibert, M. (2003). Dependence of Algal H2 Production on Photosystem II and O2 Consumption Activities in Sulfur-Deprived Chlamydomonas reinhardtii Cells. Biochimica et Biophysica Acta. Vol. 1607(2-3), 2003; pp. 153-160; NREL Report No. JA-590-35920. http://dx.doi.org/10.1016/j.bbabio.2003.09.008

78.  Kosourov, S.; Tsygankov, A.; Seibert, M.; Ghirardi, M. L. (2002). Sustained Hydrogen Photoproduction by Chlamydomonas reinhardtii: Effects of Culture Parameters. Biotechnology and Bioengineering. Vol. 78(7), 30 June 2002; pp. 731-740; NREL Report No. JA-590-30711. http://dx.doi.org/10.1002/bit.10254

79.  Makarova, V. V.; Semin, B. K.; Ghirardi, M. L.; Seibert, M. (2002). Poster 6. Chemical Modification of Arginine Residues in Photosystem II Membranes with Phenylglyoxal -- Affects on Activity (Abstract). 11th Western Photosynthesis Conference [Abstracts], 3-6 January 2002, Pacific Grove, California. pg. 22; NREL Report No. AB-590-40316.

80.  Kosourov, S.; Ghirardi, M. L.; Seibert, M. (2002). Poster 9. Influence of Extracellular pH on Hydrogen Photoproduction by Sulfur-Depleted Chlamydomonas reinhardtii Cultures (Abstract). 11th Western Photosynthesis Conference [Abstracts], 3-6 January 2002, Pacific Grove, California. pg. 33; NREL Report No. AB-590-40319.

81.  Posewitz, M. C.; Smolinski, S. L.; Zhang, L.; Melis, A.; Seibert, M.; Ghirardi, M. L. (2002). Identification of a Chlamydomonas reinhardtii Isoamylase Mutant Comprised in Hydrogen Production (Abstract). 11th Western Photosynthesis Conference [Abstracts], 3-6 January 2002, Pacific Grove, California. pg. 29; NREL Report No. AB-590-40317.

82.  Flynn, T.; Ghirardi, M. L.; Seibert, M. (2002). Accumulation of O2-Tolerant Phenotypes in H2-Producing Strains of Chlamydomonas reinhardtii by Sequential Applications of Chemical Mutagenesis and Selection. International Journal of Hydrogen Energy. Biohydrogen 2002, 21-24 April 2002, Ede, The Netherlands. Vol. 27(11-12), 2002; pp. 1421-1430; NREL Report No. JA-590-33327. http://dx.doi.org/10.1016/S0360-3199(02)00117-9

83.  Semin, B. K.; Ghirardi, M. L.; Seibert, M. (2002). Blocking of Electron Donation by Mn(II) to Yz* Following Incubation of Mn-Depleted Photosystem II Membranes with Fe(II) in the Light. Biochemistry. Vol. 41(18), 2002; pp. 5854-5864; NREL Report No. JA-590-32609. http://dx.doi.org/10.1021/bi0200054

84.  Plummer, S. M; Ghirardi, M. L.; Ahmann, D. (2002). Poster 11. Biologically Generated Hydrogen Utilizing a Photosynthetic Green Alga (Abstract). 11th Western Photosynthesis Conference [Abstracts], 3-6 January 2002, Pacific Grove, California. pg. 34; NREL Report No. AB-590-40320.

85.  Laurinavichene, T. V.; Tolstygina, I. V.; Galiulina, R. R.; Ghirardi, M. L.; Seibert, M.; Tsygankov, A. A. (2002). Dilution Methods to Deprive Chlamydomonas reinhardtii Cultures of Sulfur for Subsequent Hydrogen Photoproduction. International Journal of Hydrogen Energy. Biohydrogen 2002, 21-24 April 2002, Ede, The Netherlands. Vol. 27(11-12), 2002; pp. 1245-1249; NREL Report No. JA-590-33325. http://dx.doi.org/10.1016/S0360-3199(02)00101-5

86.  Seibert, M.; King, P.; Zhang, L.; Mets, L.; Ghirardi, M. (2002). Molecular Engineering of Algal H2 Production. Proceedings of the 2002 U.S. DOE Hydrogen and Fuel Cells Annual Program/Lab R&D Review, 6-10 May 2002, Golden, Colorado (NREL/CP-610-32405 CD-ROM). Golden, CO: National Renewable Energy Laboratory pp. 20-29; NREL Report No. CP-590-32408.

87.  Ghirardi, M. L.; Kosourov, S.; Tsygankov, A.; Rubin, A.; Seibert, M. (2002). Cyclic Photobiological Algal H2-Production. Proceedings of the 2002 U.S. DOE Hydrogen and Fuel Cells Annual Program/Lab R&D Review, 6-10 May 2002, Golden, Colorado (NREL/CP-610-32405 CD-ROM). Golden, CO: National Renewable Energy Laboratory pp. 52-63; NREL Report No. CP-590-32409.

88.  Zhang, L.; King, P.; Seibert, M.; Ghirardi, M. L. (2002). Poster 19. Direct Evidence for Different Electron Transport Pathways Linked to H2 Production in the Green Alga, Chlamydomonas reinhardtii (Abstract). 11th Western Photosynthesis Conference [Abstracts], 3-6 January 2002, Pacific Grove, California. pg. 44; NREL Report No. AB-590-40321.

89.  Tsygankov, A.; Kosourov, S.; Seibert, M.; Ghirardi, M. L. (2002). Hydrogen Photoproduction under Continuous Illumination by Sulfur-Deprived, Synchronous Chlamydomonas reinhardtii Cultures. International Journal of Hydrogen Energy. Biohydrogen 2002, 21-24 April 2002, Ede, The Netherlands. Vol. 27(11-12), 2002; pp. 1239-1244; NREL Report No. JA-590-33324. http://dx.doi.org/10.1016/S0360-3199(02)00108-8

90.  Ghirardi, M. L.; Kosourov, S.; Seibert, M. (2001). Cyclic Photobiological Aglal H2-Production. Proceedings of the 2001 U.S. DOE Hydrogen Program Review, 17-19 April 2001, Baltimore, Maryland. NREL/CP-610-30535. Golden, CO: National Renewable Energy Laboratory pp. 67-76; NREL Report No. CP-610-32309.

91.  Seibert, M.; Flynn, T. Y.; Ghirardi, M. L. (2001). Strategies for Improving Oxygen Tolerance of Algal Hydrogen Production. Miyake, J.; Matsunaga, T.; San Pietro, A., eds. Biohydrogen II. Oxford, UK: Pergamon pp. 67-77; NREL Report No. CP-590-31874.

92.  Ghirardi, M. L.; Kosourov, S.; Seibert, M. (2001). Optimization of a Cyclic Algal Hydrogen Photoproduction System for Possible Future Commercial Applications (Viewgraphs only). McLean, G. F., ed. Building the Hydrogen Economy: Proceedings of the 11th Canadian Hydrogen Conference, 17-20 June 2001, Victoria, British Colombia, Canada. Toronto: Canadian Hydrogen Association Vol 2: pp. 504-510; NREL Report No. CP-590-33356.

93.  Seibert, M.; Forestier, M.; Zhang, L.; Ghirardi, M. (2001). Development of an Efficient Algal H2-Production System. Proceedings of the 2001 U.S. DOE Hydrogen Program Review, 17-19 April 2001, Baltimore, Maryland. NREL/CP-610-30535. Golden, CO: National Renewable Energy Laboratory pp. 23-30; NREL Report No. CP-610-32308.

94.  Melis, A.; Zhang, L.; Forestier, M.; Ghirardi, M. L.; Seibert, M. (2000). Sustained Photobiological Hydrogen Gas Production upon Reversible Inactivation of Oxygen Evolution in the Green Alga Chlamydomonas reinhardtii. Plant Physiology. Vol. 122, January 2000; pp. 127-135; NREL Report No. 28199. http://dx.doi.org/10.1104/pp.122.1.127

95.  Ghirardi, M.; Flynn, T.; Forestier, M.; Seibert, M. (2000). Development of an Efficient Algal H2-Production System. Proceedings of the 1999 U.S. DOE Hydrogen Program Review, 4-6 May 1999, Lakewood, Colorado. NREL/CP-570-26938. Golden, CO: National Renewable Energy Laboratory Vol. I: pp. 16-29; NREL Report No. CP-570-32259.

96.  Ghirardi, M. L.; Zhang, L.; Lee, J. W.; Flynn, T.; Seibert, M.; Greenbaum, E.; Melis, A. (2000). Microalgae: A Green Source of Renewable H2. Trends in Biotechnology. Vol. 18, December 2000; pp. 506-511; NREL Report No. JA-590-30055. http://dx.doi.org/10.1016/S0167-7799(00)01511-0

97.  Melis, A.; Zhang, L.; Forestier, M.; Ghirardi, M. L.; Seibert, M. (2000). Sustained Photobiological Hydrogen Gas Production Upon Reversible Inactivation of Oxygen in the Green Alga Chlamydomonas reinhardtii. Proceedings of the 1999 U.S. DOE Hydrogen Program Review, 4-6 May 1999, Lakewood, Colorado. NREL/CP-570-26938. Golden, CO: National Renewable Energy Laboratory Vol. I: pp. 40-58; NREL Report No. CP-570-32261.

98.  Ghirardi, M. L.; Huang, Z.; Forestier, M.; Smolinski, S.; Posewitz, M.; Seibert, M. (2000). Development of an Efficient Algal H2-Producing System. Proceedings of the 2000 U.S. DOE Hydrogen Program Review, 9-11 May 2000, San Ramon, California. NREL/CP-570-28890. Golden, CO: National Renewable Energy Laboratory Vol. I: pp. 272-281; NREL Report No. CP-570-32299.

99.  Ghirardi, M. L.; Kosourov, S.; Tsygankov, A.; Seibert, M. (2000). Two-Phase Photobiological Algal H2-Production System. Proceedings of the 2000 U.S. DOE Hydrogen Program Review, 9-11 May 2000, San Ramon, California. NREL/CP-570-28890. Golden, CO: National Renewable Energy Laboratory Vol. I: pp. 282-294; NREL Report No. CP-570-32300.

100.  Flynn, T.; Ghirardi, M. L.; Seibert, M. (1999). Isolation of Chlamydomonas Hydrogenase Mutants with Improved Oxygen Tolerance. (Abstract 086-FUEL). American Chemical Society. Abstracts of Papers of the 218th ACS National Meeting, 22-26 August 1999, New Orleans, Louisiana. Washington, DC: American Chemical Society 1 p.; NREL Report No. AB-510-35833.

101.  Flynn, T.; Ghirardi, M. L.; Seibert, M. (1999). Isolation of Chlamydomonas Mutants with Improved Oxygen-Tolerance. Division of Fuel Chemistry, American Chemical Society: Preprints of Symposia, 218th ACS National Meeting, 22-26 August 1999, New Orleans, Louisiana. Vol. 44, No. 4. Washington, DC: American Chemical Society pp. 846-850; NREL Report No. CP-29039.

102.  Ghirardi, M. L.; Seibert, M. (1999). Process for Selection of Oxygen-Tolerant Alagal Mutants that Produce H2 under Aerobic Conditions. U.S. Patent No. 5,871,952. 11 pp.; NREL Report No. 26411.

103.  Seibert, M.; Flynn, T.; Benson, D.; Tracy, E.; Ghirardi, M. (1998). Development of Selection and Screening Procedures for Rapid Identification of H2-Producing Algal Mutants with Increased O2 Tolerance. Zaborsky, O. R., et al., eds. BioHydrogen: Proceedings of an International Conference on Biological Hydrogen Production, 23-26 June 1997, Waikoloa, Hawaii. New York: Plenum Press pp. 227-234; NREL Report No. CP-450-23325.

104.  Ghirardi, M. L.; Flynn, T.; Forestier, M.; Seibert, M. (1998). Development of an Efficient Algal H2-Production System. Proceedings of the 1998 U.S. DOE Hydrogen Program Review, 28-30 April 1998, Alexandria, Virginia. NREL/CP-570-25315. Golden, CO: National Renewable Energy Laboratory Vol. I: pp. 43-60; NREL Report No. 25663.

105.  Ghirardi, M. L.; Flynn, T.; Forestier, M.; Iyer, A.; Melis, A.; Danielson, P.; Seibert, M. (1998). Generation of C. reinhardtii Mutants That Photoproduce H2 from H2O in the Presence of O2. Garab, G., ed. Photosynthesis: Mechanisms and Effects. Proceedings of the 11th International Congress on Photosynthesis, 17-22 August 1998, Budapest, Hungary. Dordrecht: Kluwer Academic Publishers Vol. III; pp. 1959-1962; NREL Report No. CP-590-29079.

106.  Ghirardi, M. L.; Lutton, T. W.; Seibert, M. (1998). Effects of Carboxyl Amino Acid Modification on the Properties of the High-Affinity, Manganese-Binding Site in Photosystem II. Biochemistry. Vol. 37, 1998; pp. 13559-13566; NREL Report No. JA-590-24164. http://dx.doi.org/10.1021/bi980358w

107.  Ghirardi, M. L.; Preston, C.; Seibert, M. (1998). Use of a Novel Histidyl Modifier to Probe on Tris-Treated Photosystem II Membrane Fragments That May Bind Functional Manganese. Biochemistry. Vol. 37, 1998; pp. 13567-13574; NREL Report No. JA-590-24165. http://dx.doi.org/10.1021/bi980359o

108.  Ghirardi, M.; Seibert, M. (1997). Techniques for Isolation of Oxygen-Tolerant Mutants of Hydrogen-Producing Chlamydomonas reinhardtii. Supplement to Plant Physiology: Plant Biology '97 Final Program. Final Program and Abstracts of the 1997 Annual Meetings of American Society of Plant Physiologists and the Canadian Society of Plant Physiologists, 2-6 August 1997, Vancouver, British Columbia, Canada. Vol. 114(3), July 1997; p. 205; NREL Report No. 21453.

109.  Ghirardi, M. L.; Togasaki, R. K.; Seibert, M. (1997). Oxygen Sensitivity of Algal H2-Production. Applied Biochemistry and Biotechnology. Proceedings of the Eighteenth Symposium on Biotechnology for Fuels and Chemicals, 5-9 May 1996, Gatlinburg, Tennessee. Vol. 63-65, Spring 1997; pp. 141-151; NREL Report No. TP-450-21371. http://dx.doi.org/10.1007/BF02920420

110.  Ghirardi, M. L.; Seibert, M. (1997). Isolation of Oxygen-Tolerant, Hydrogen-Producing Mutants fro Chlamydomonas Reinhardtii: Approaches and Preliminary Results. Photochemistry and Photobiology: Abstracts of the 25th Annual Meeting of the American Society for Photobiology, 5-10 July 1997, St. Louis, Missouri. p. 11S; NREL Report No. 24269.

111.  Ghirardi, M. L.; Flynn, T.; Markov, S.; Seibert, M. (1997). Development of an Efficient Algal H2-Production System. Proceedings of the 1997 U.S. DOE Hydrogen Program Review, 21-23 May 1997, Herndon, Virginia. NREL/CP-430-23722. Golden, CO: National Renewable Energy Laboratory pp. 11-24; NREL Report No. 25593.

112.  Ghirardi, M. L.; Lutton, T. W.; Seibert, M. (1996). Interactions Between Diphenylcarbazide, Zinc, Cobalt, and Manganese on the Oxidizing Side of Photosystem II. Biochemistry. Vol. 35(6), 19960000; pp. 1820-1828; NREL Report No. TP-452-7489. http://dx.doi.org/10.1021/bi951657d

113.  Ghirardi, M. L.; Markov, S.; Seibert, M. (1996). Development of an Efficient Algal H2-Producing System. Proceedings of the 1996 U.S. DOE Hydrogen Program Review, 1-2 May 1996, Miami, Florida. NREL/CP-430-21968. Vol. I: pp. 285-302; NREL Report No. 22543.

114.  Ghirardi, M. L.; Toon, S. P.; Seibert, M. (1995). Development of an Efficient Algal H2-Producing System. Proceedings of the 1995 U.S. DOE Hydrogen Program Review, 18-21 April 1995, Coral Gables, Florida. NREL/CP-430-20036. Golden, CO: National Renewable Energy Laboratory Vol. 2: pp. 683-692.d; NREL Report No. 20671.

115.  Toon, S. P.; Ghirardi, M.; Mohanty, P.; Seibert, M. (1995). Cadmium Inhibition of O2 Evolution and Electron Transport in Isolated Photosystem II Membranes. (Abstract 425). Plant Physiology (Supplement). Abstracts of the 1995 Annual Meeting of the American Society of Plant Physiologists, 29 July - 2 August 1995, Charlotte, North Carolina. Vol. 108(2), June 1995; p. 89; NREL Report No. 20085.

116.  Ghirardi, M. L.; Lutton, T. W.; Seibert, M. (1995). Selective Effect of Carboxyl and Histidyl Modifiers on Binding and Single Turnover Photooxidation of Mn2+ by Photosystem II. Mathis, P., ed. Photosynthesis: from Light to Biosphere - Proceedings of the 10th International Photosynthesis Congress, 20-25 August 1995, Montpellier, France. Dordrecht, Netherlands: Kluwer Academic Publishers Vol. II: pp. 409-412; NREL Report No. 21012.

117.  Ghirardi, M. L.; Seibert, M. (1992). Electron Donation to Photosystem II by Manganese and Diphenylcarbazide-Kinetic Parameters. Murata, N., ed. Research in Photosynthesis: Proceedings of the IXth International Congress on Photosynthesis, 30 August-4 September 1992, Nagoya, Japan. Dordrect, Netherlands: Kluwer Academic Publishers Vol II: pp. 357-360; NREL Report No. TP-452-4884.

118.  Ghirardi, M. L.; Seibert, M. (1992). Electron Donation to Photosystem II by Manganese and Diphenylcarbazide - Kinetic Parameters. (Abstract P-235). Photosynthesis Research. Vol. 34(1), 1992; p. 147.



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