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Records 1 to 54 of 54

1.  King, P.; Ghirardi, M. L.; Seibert, M. (2014). Oxygen-Resistant Hydrogenases and Methods for Designing and Making Same. U.S. Patent No. 8,663,958.

2.  Brown, K. A.; Song, Q.; Mulder, D. W.; King, P. W. (2014). Diameter Dependent Electron Transfer Kinetics in Semiconductor-Enzyme Complexes. ACS Nano. Vol. 8(10), 28 October 2014; pp. 10790-10798; NREL Report No. JA-2700-62598. http://dx.doi.org/10.1021/nn504561v

3.  Seibert, M.; King, P. W.; Ghirardi, M. L.; Posewitz, M. C.; Smolinski, S. L. (2014). Process and Genes for Expression and Overexpression of Active [FeFe] Hydrogenases. U.S. Patent No. 8,835,153.

4.  Long, H.; King, P. W.; Chang, C. H. (2014). Proton Transport in Clostridium pasteurianum (FeFe) Hydrogenase I: A Computational Study. Journal of Physical Chemistry B. Vol. 118(4), 30 January 2014; pp. 890-900; NREL Report No. JA-2C00-60227. http://dx.doi.org/10.1021/jp408621r

5.  Wilker, M. B.; Shinopoulos, K. E.; Brown, K. A.; Mulder, D. W.; King, P. W.; Dukovic, G. (2014). Electron Transfer Kinetics in CdS Nanorod-[FeFe]-Hydrogenase Complexes and Implications for Photochemical H2 Generation. Journal of the American Chemical Society. Vol. 136(11), 19 March 2014; pp. 4316-4324; NREL Report No. JA-2700-61588. http://dx.doi.org/10.1021/ja413001p

6.  Mulder, D. W.; Ratzloff, M. W.; Bruschi, M.; Greco, C.; Koonce, E.; Peters, J. W.; King, P. W. (2014). Investigations on the Role of Proton-Coupled Electron Transfer in Hydrogen Activation by [FeFe]-Hydrogenase. Journal of the American Chemical Society. Vol. 136(43), 29 October 2014; pp. 15394-15402; NREL Report No. JA-2700-62666. http://dx.doi.org/10.1021/ja508629m

7.  King, P. W. (2013). Designing Interfaces of Hydrogenase-Nanomaterial Hybrids for Efficient Solar Conversion. Biochimica et Biophysica Acta (BBA) - Bioenergetics. Vol. 1827(8-9), August-September 2013; pp. 949-957; NREL Report No. JA-2700-58365. http://dx.doi.org/10.1016/j.bbabio.2013.03.006

8.  Mulder, D. W.; Ratzloff, M. W.; Shepard, E. M.; Byer, A. S.; Noone, S. M.; Peters, J. W.; Broderick, J. B.; King, P. W. (2013). EPR and FTIR Analysis of the Mechanism of H2 Activation by [FeFe]-Hydrogenase HydA1 from Chlamydomonas reinhardtii. Journal of the American Chemical Society. Vol. 135(18), 8 May 2013; pp. 6921-6929; NREL Report No. JA-2700-57594. http://dx.doi.org/10.1021/ja4000257

9.  Peden, E. A.; Boehm, M.; Mulder, D. W.; Davis, R.; Old, W. M.; King, P. W.; Ghirardi, M. L.; Dubini, A. (2013). Identification of Global Ferredoxin Interaction Networks in Chlamydomonas reinhardtii. Journal of Biological Chemistry. Vol. 288(49), 6 December 2013; pp. 35192-35209; NREL Report No. JA-2700-60563. http://dx.doi.org/10.1074/jbc.M113.483727

10.  Moore, T. A.; Moore, A. L.; Gust, D.; Madden, C.; Vaughn, M. D.; King, P. W.; Brown, K. A.; Diez-Perez, I. (2012). Catalytic Turnover of [FeFe]-Hydrogenase Based on Single Molecule Imaging. Abstract No. FUEL-289. American Chemical Society. Abstracts of Papers of the 243rd ACS National Meeting, 25-29 March 2012, San Diego, California. Washington, DC: American Chemical Society (ACS) 1 pg.; NREL Report No. AB-2700-56589.

11.  Yacoby, I.; Tegler, L. T.; Pochekailov, S.; Zhang, S.; King, P. W. (2012). Optimized Expression and Purification for High-Activity Preparations of Algal [FeFe]-Hydrogenase. Article No. e35886. PLoS One. Vol. 7(4), April 2012; 8 pp.; NREL Report No. JA-2700-52560. http://dx.doi.org/10.1371/journal.pone.0035886

12.  Madden, C.; Vaughn, M. D.; Diez-Perez, I.; Brown, K. A.; King, P. W.; Gust, D.; Moore, A. L.; Moore, T. A. (2012). Catalytic Turnover of [FeFe]-Hydrogenase Based on Single Molecule Imaging. Journal of the American Chemical Society. Vol. 134(3), 25 January 2012; pp. 1577-1582; NREL Report No. JA-2700-52561. http://dx.doi.org/10.1021/ja207461t

13.  Brown, K. A.; Wilker, M. B.; Boehm, M.; Dukovic, G.; King, P. W. (2012). Characterization of Photochemical Processes for H2 Production by CdS Nanorod-[FeFe] Hydrogenase Complexes. Journal of the American Chemical Society. Vol. 134(12), 28 March 2012; pp. 5627-5636; NREL Report No. JA-2700-53672. http://dx.doi.org/10.1021/ja2116348

14.  King, P. W.; Brown, K. A.; Ratcliff, K.; Beer, L. L. (2012). Chapter 11: Photobiological and Photobiomimetic Production of Solar Fuels. Muradov, N. Z. and Veziroglu, T., eds. Carbon-Neutral Fuels and Energy Carriers. Green Chemistry and Chemical Engineering Series. Boca Raton, FL: CRC Press pp. 547-602; NREL Report No. CH-2700-49502.

15.  Eckert, C.; Dubini, A.; Yu, J.; King, P.; Seibert, M.; Maness, P. C. (2012). Hydrogenase Genes and Enzymes Involved in Solar Hydrogen Production. Azbar, N. and Levin, D. B., eds. State of the Art and Progress in Production of Biohydrogen. Sharjah, U.A.E.: Bentham Science Publishers pp. 8-24; NREL Report No. JA-270-48161. http://dx.doi.org/10.2174/978160805224011201010008

16.  Morra, S.; Giraudo, A.; Di Nardo, G.; King, P. W.; Gilardi, G.; Valetti, F. (2012). Site Saturation Mutagenesis Demonstrates a Central Role for Cysteine 298 as Proton Donor to the Catalytic Site in CaHydA [FeFe]-Hydrogenase. Article No. e48400. PLoS One. Vol. 7(10), October 2012; 8 pp.; NREL Report No. JA-2700-54969. http://dx.doi.org/10.1371/journal.pone.0048400

17.  Svedruzic, D.; Blackburn, J. L.; Tenent, R. C.; Rocha, J. D. R.; Vinzant, T. B.; Heben, M. J.; King, P. W. (2011). High-Performance Hydrogen Production and Oxidation Electrodes with Hydrogenase Supported on Metallic Single-Wall Carbon Nanotube Networks. Journal of the American Chemical Society. Vol. 133(12), 30 March 2011; pp. 4299-4306; NREL Report No. JA-2700-48387. http://dx.doi.org/10.1021/ja104785e

18.  King, P. W.; Brown, K. A.; Beernik, M. B.; Dukovic, G.; Rumbles, G.; Dayal, S. (2011). Hybrid Molecular Assemblies for Solar Hydrogen Production. Abstract No. FUEL-270. American Chemical Society. Abstracts of Papers of the 242nd ACS National Meeting, 28 August - 1 September 2011, Denver, Colorado. Washington, DC: American Chemical Society (ACS) 1 pg.; NREL Report No. AB-2700-53211.

19.  Morra, S.; Valetti, F.; Sadeghi, S. J.; King, P. W.; Meyer, T.; Gilardi, G. (2011). Direct Electrochemistry of a [FeFe]-Hydrogenase on TiO2 Electrode. Chemical Communications. Vol. 47(38), 14 October 2011; pp. 10566-10568; NREL Report No. JA-2700-52075. http://dx.doi.org/10.1039/C1CC14535E

20.  Yacoby, I.; Pochekailov, S.; Toporik, H.; Ghirardi, M. L.; King, P. W.; Zhang, S. G. (2011). Photosynthetic Electron Partitioning Between [FeFe] Hydrogenase and Ferredoxin: NADP+-Oxidoreductase (FNR) Enzymes in Vitro. Proceedings of the National Academy of Sciences of the United States of America. Vol. 108(23), 7 June 2011; pp. 9396-9401; NREL Report No. JA-2700-51092. http://dx.doi.org/10.1073/pnas.1103659108

21.  Mulder, D. W.; Shepard, E. M.; Meuser, J. E.; Joshi, N.; King, P. W.; Posewitz, M. C.; Broderick, J. B.; Peters, J. W. (2011). Insights into [FeFe]-Hydrogenase Structure, Mechanism, and Maturation. Structure. Vol. 19(8), 10 August 2011; pp. 1038-1052; NREL Report No. JA-2700-51320. http://dx.doi.org/10.1016/j.str.2011.06.008

22.  Brown, K. A.; Dayal, S.; Ai, X.; Rumbles, G.; King, P. W. (2010). Controlled Assembly of Hydrogenase-CdTe Nanocrystal Hybrids for Solar Hydrogen Production. Journal of the American Chemical Society. Vol. 132(28), 2010; pp. 9672-9680; NREL Report No. JA-270-47560. http://dx.doi.org/10.1021/ja101031r

23.  Long, H.; King, P. W.; Ghirardi, M. L.; Kim, K. (2009). Hydrogenase/Ferredoxin Charge-Transfer Complexes: Effect of Hydrogenase Mutations on the Complex Association. Journal of Physical Chemistry A. Vol. 113(16), 2009; pp. 4060-4067; NREL Report No. JA-590-44322. http://dx.doi.org/10.1021/jp810409z

24.  King, P.; Ghirardi, M. L.; Seibert, M. (2009). Oxygen-Resistant Hydrogenases and Methods for Designing and Making Same. U.S. Patent No. 7,501,270 B2. 23 pp.; NREL Report No. PT-270-45830.

25.  English, C. M.; Eckert, C.; Brown, K.; Seibert, M.; King, P. W. (2009). Recombinant and In Vitro Expression Systems for Hydrogenases: New Frontiers in Basic and Applied Studies for Biological and Synthesis H2 Production. Dalton Transactions. (45), 2009; pp. 9970-9978; NREL Report No. JA-2A0-46882. http://dx.doi.org/10.1039/b913426n

26.  Long, H.; Chang, C. H.; King, P. W.; Ghirardi, M. L.; Kim, K. (2008). Brownian Dynamics and Molecular Dynamics Study of the Association Between Hydrogenase and Ferredoxin from the Chlamydomonas reinhardtii. Biophysical Journal. Vol. 95, October 2008; pp. 3753-3766; NREL Report No. JA-530-41890. http://dx.doi.org/10.1529/biophysj.107.127548

27.  Seibert, M.; King, P. W.; Posewitz, M. C.; Melis, A.; Ghirardi, M. L. (2008). Chapter 22: Photosynthetic Water-Splitting for Hydrogen Production. Wall, J. D., Harwood, C. S., Demain, A., eds. Bioenergy. Washington, D.C.: ASM Press pp. 273-291; NREL Report No. JA-270-42871.

28.  Svedruzic-Chang, D.; Blackburn, J. L.; McDonald, T. J.; Heben, M. J.; King, P. W. (2008). Studies of Hybrid Nano-Bio-System: Single-Walled Carbon Nanotubes and Hydrogenase. NSTI-Nanotech 2008: Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, 1-5 June 2008, Boston, Massachusetts. Philadelphia, PA: Taylor and Francis Vol. 2: pp. 289-292; NREL Report No. CP-270-43005.

29.  Blackburn, J. L. Svedruzic, D.; McDonald, T. J.; Kim, Y. H.; King, P. W.; Heben, M. J. (2008). Raman Spectroscopy of Charge Transfer Interactions Between Single Wall Carbon Nanotubes and [FeFe] Hydrogenase. Dalton Transactions. (40), 2008; pp. 5454-5461; NREL Report No. JA-590-44674. http://dx.doi.org/10.1039/b806379f

30.  King, P. W.; Heben, M. J. (2008). Biohybrid Catalysis for Solar Hydrogen Production. SPIE Newsroom. , 14 January 2008; 2 p.; NREL Report No. JA-270-42598. http://dx.doi.org/10.1117/2.1200801.0990

31.  Hambourger, M.; Gervaldo, M.; Svedruzic, D.; King, P. W.; Gust, D.; Ghirardi, M.; Moore, A. L.; Moore, T. A. (2008). [FeFe]-Hydrogenase-Catalyzed H2 Production in a Photoelectrochemical Biofuel Cell. Journal of the American Chemical Society. Vol. 130(6), 2008; pp. 2015-2022; NREL Report No. JA-270-42429. http://dx.doi.org/10.1021/ja077691k

32.  King, P. W.; Svedruzic, D.; Hambourger, M.; Gervaldo, M.; McDonald, T.; Blackburn, J.; Heben, M.; Gust, D.; Moore, A. L.; Moore, T. A.; Ghirardi, M. L. (2007). Merging [FeFe]-Hydrogenases with Materials and Nanomaterials as Biohybrid Catalysts for Solar H2 Production. Guo, J. ed. Solar Hydrogen and Nanotechnology II. Proceedings of SPIE, Vol. 6650. 7 pp.; NREL Report No. CP-270-42088. http://dx.doi.org/10.1117/12.736556

33.  Chang, D. S.; McDonald, T. J.; Kim, Y.-H.; Blackburn, J. L.; Heben, M. J.; King, P. W. (2007). Interaction of [FeFe]-Hydrogenases with Single-walled Carbon Nanotubes. Guo, J. ed. Solar Hydrogen and Nanotechnology II. Proceeding of SPIE, Vol. 6550. 9 pp.; NREL Report No. CP-270-42219. http://dx.doi.org/10.1117/12.736232

34.  Chang, C. H.; King, P. W.; Ghirardi, M. L.; Kim, K. (2007). Atomic Resolution Modeling of the Ferredoxin:[FeFe] Hydrogenase Complex from Chlamydomonas reinhardtii. Biophysical Journal. Vol. 93, November 2007; pp. 3034-3045; NREL Report No. JA-530-41713. http://dx.doi.org/10.1529/biophysj.107.108589

35.  McDonald, T. J.; Svedruzic, D.; Kim, Y. H.; Blackburn, J. L.; Zhang, S. B.; King, P. W.; Heben, M. J. (2007). Wiring-Up Hydrogenase with Single-Walled Carbon Nanotubes. Nano Letters. Vol. 7(11), 2007; pp. 3528-3534; NREL Report No. JA-270-42272. http://dx.doi.org/10.1021/nl072319o

36.  McGlynn, S. E.; Ruebush, S. S.; Naumov, A.; Nagy, L. E.; Dubini, A.; King, P. W.; Broderick, J. B.; Posewitz, M. C.; Peters, J. W. (2007). In Vitro Activation of [FeFe] Hydrogenase: New Insights into Hydrogenase Maturation. Journal of Biological Inorganic Chemistry. Vol. 12, 2007; pp. 443-447; NREL Report No. JA-270-41769. http://dx.doi.org/10.1007/s00775-007-0224-z

37.  Nagy, L. E.; Meuser, J. E.; Plummer, S.; Seibert, M.; Ghirardi, M. L.; King, P. W.; Ahmann, D.; Posewitz, M. C. (2007). Application of Gene-Shuffling for the Rapid Generation of Novel [FeFe]-Hydrogenase Libraries. Biotechnology Letters. Vol. 29(3), 2007; pp. 421-430; NREL Report No. JA-270-41455. http://dx.doi.org/10.1007/s10529-006-9254-9

38.  King, P. W.; Svedruzic, D.; Cohen, J.; Schulten, K.; Seibert, M.; Ghirardi, M. L. (2006). Structural and Functional Investigations of Biological Catalysts for Optimization of Solar-Driven, H2 Production Systems. Vayssieres, L. Solar Hydrogen and Nanotechnology. Proceedings of SPIE, Vol. 6340. Vol. 6340, 2006; Bellingham, WA: SPIE 9 pp.; NREL Report No. CP-270-44067. http://dx.doi.org/10.1117/12.693636;

39.  Bock, A.; King, P. W.; Blokesch, M.; Posewitz, M. C. (2006). Maturation of Hydrogenases. Poole, R. K., ed. Advances in Microbial Physiology, Vol. 51. New York: Elsevier Ltd. pp. 1-71; NREL Report No. CH-270-41764.


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40.  Ghirardi, M.; King, P.; Maness, P. C.; Seibert, M. (2006). Biological Systems for Hydrogen Photoproduction (Poster). 24 pp.; NREL Report No. PO-560-39994.

41.  Ghirardi, M. L.; Cohen, J.; King, P.; Schulten, K.; Kim, K.; Seibert, M. (2006). [FeFe]-Hydrogenases and Photobiological Hydrogen Production. Vayssieres, L. Solar Hydrogen and Nanotechnology. Proceedings of SPIE, Vol. 6340. Vol. 6340, 2006; Bellingham, WA: SPIE 6 pp.; NREL Report No. CP-270-44065. http://dx.doi.org/10.1117/12.683767;

42.  King, P. W.; Posewitz, M. C.; Ghirardi, M. L.; Seibert, M. (2006). Functional Studies of [FeFe] Hydrogenase Maturation in an Escherichia coli Biosynthetic System. Journal of Bacteriology. Vol. 188(6), March 2006; pp. 2163-2172; NREL Report No. JA-590-40255. http://dx.doi.org/10.1128/JB.188.6.2163-2172.2006

43.  Ghirardi, M. L.; King, P.; Seibert, M. (2006). Chapter II.E.2: Biological Systems for Hydrogen Photoproduction. DOE Hydrogen Program 2006 Annual Progress Report. pp. 120-124; NREL Report No. CH-270-40266.

44.  Ghirardi, M. L.; King, P.; Kosourov, S.; Forestier, M.; Zhang, L.; Seibert, M. (2005). Chapter 11: Development of Algal Systems for Hydrogen Photoproduction; Addressing the Hydrogenase Oxygen-Sensitivity Problem. Collings, A. F.; Critchley, C. eds. Artificial Photosynthesis: From Basic Biology to Industrial Application. Weinheim, Germany: Wiley-VCH GmbH & Co. KGaA pp. 213-227; NREL Report No. CH-590-40692.

45.  Cohen, J.; Kim, K.; Posewitz, M.; Ghirardi, M. L.; Schulten, K.; Seibert, M.; King, P. (2005). Molecular Dynamics and Experimental Investigation of H2 and O2 Diffusion in [Fe]-Hydrogenase. Biochemical Society Transactions. Vol. 33(Part 1), 2005; pp. 80-82; NREL Report No. JA-590-38440.

46.  Posewitz, M. C.; King, P. W.; Smolinski, S. L.; Smith II, R. D.; Ginley, A. R.; Ghirardi, M. L.; Seibert, M. (2005). Identification of Genes Required for Hydrogenase Activity in Chlamydomonas reinhardtii. Biochemical Society Transactions. Vol. 33(Part 1), 2005; pp. 102-104; NREL Report No. JA-590-38657.


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47.  Ghirardi, M. L.; Kim, K.; King, P.; Maness, P. C.; Seibert, M. (2005). Biological Systems for Hydrogen Photoproduction (Presentation). 20 pp.; NREL Report No. PR-560-38183.

48.  Ghirardi, M. L.; King, P. W.; Posewitz, M. C.; Maness, P. C.; Fedorov, A.; Kim, K.; Cohen, J.; Schulten, K.; Seibert, M. (2005). Approaches to Developing Biological H2-Photoproducing Organisms and Processes. Biochemical Society Transactions. Vol. 33(Part 1), 2005; pp. 70-72; NREL Report No. JA-590-38437.

49.  Cohen, J.; Kim, K.; King, P.; Seibert, M.; Schulten, K. (2005). Finding Gas Diffusion Pathways in Proteins: Application to O2 and H2 Transport in Cpl [FeFe]-Hydrogenase and the Role of Packing Defects. Structure. Vol. 13(9), September 2005; pp. 1321-1329; NREL Report No. JA-530-39361. http://dx.doi.org/10.1016/j.str.2005.05.013

50.  Posewitz, M. C.; King, P. W.; Smolinski, S. L.; Zhang, L.; Seibert, M.; Ghirardi, M. L. (2004). Discovery of Two Novel Radical S-Adenosylmethionine Proteins Required for the Assembly of an Active [Fe] Hydrogenase. Journal of Biological Chemistry. Vol. 279(24), 11 June 2004; pp. 25711-25720; NREL Report No. JA-590-36919. http://dx.doi.org/10.1074/jbc.M403206200


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51.  Kim, K.; Jones, W.; Himmel, M.; King, P. (2004). Abstracts for the Workshop on Computational Biology: Focus on Hydrogen, Biomass, and Nanoscience. 28 pp.; NREL Report No. BK-530-36724.

52.  Forestier, M.; King, P.; Zhang, L.; Posewitz, M.; Schwarzer, S.; Happe, T.; Ghirardi, M. L.; Seibert, M. (2003). Expression of two [Fe]-Hydrogenases in Chlamydomonas reinhardtii under Anaerobic Conditions. European Journal of Biochemistry. Vol. 270, 2003; pp. 2750-2758; NREL Report No. JA-590-34980. http://dx.doi.org/10.1046/j.1432-1033.2003.03656

53.  Seibert, M.; King, P.; Zhang, L.; Mets, L.; Ghirardi, M. (2002). Molecular Engineering of Algal H2 Production. Proceedings of the 2002 U.S. DOE Hydrogen and Fuel Cells Annual Program/Lab R&D Review, 6-10 May 2002, Golden, Colorado (NREL/CP-610-32405 CD-ROM). Golden, CO: National Renewable Energy Laboratory pp. 20-29; NREL Report No. CP-590-32408.

54.  Zhang, L.; King, P.; Seibert, M.; Ghirardi, M. L. (2002). Poster 19. Direct Evidence for Different Electron Transport Pathways Linked to H2 Production in the Green Alga, Chlamydomonas reinhardtii (Abstract). 11th Western Photosynthesis Conference [Abstracts], 3-6 January 2002, Pacific Grove, California. pg. 44; NREL Report No. AB-590-40321.



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